Book Description

Written by active scientists, this book provides readers with a logical framework for conducting modern biochemical and molecular biology research. The authors write with passion, and their love of biochemical research shines through on every page. ... Read more

Book Description

Organic Structure Analysis is an ideal text for upper-level undergraduate and first-year graduate courses in organic spectroscopy. It provides students with the background necessary to effectively apply spectroscopic data in establishing features of molecular structure. Unique in its coverage, it is the only text that emphasizes strategies, interpretation, modern techniques, and problem solving. The text's major aim is to show how spectra can be efficiently examined in order to identify a molecule's major structural elements. It focuses on four techniques which are used on a day-to-day basis by most organic chemists: nuclear magnetic resonance (NMR), mass spectroscopy (MS), infrared (IR), and ultraviolet-visible (UV-VIS). Each chapter provides different perspectives on using spectroscopic data in a step-by-step process to establish structural features such as an overall carbon framework; the presence and location of functional groups; the regiochemistry and stereochemistry of substituents; and, finally, the absolute stereochemistry of chiral centers. Recent advances in NMR and MS have changed the way these components are introduced and used in organic spectroscopy courses. Unlike similar texts, this book provides ample, up-to-date coverage of the latest developments in NMR techniques.

Organic Structure Analysis adopts a practical approach to the subject which emphasizes building experience; the authors recognize that a combination of problem solving, access to data from models, and understanding the rules of spectral interpretation helps students to build expertise in structure determination from spectra. It outlines elements of theory, but with an emphasis on those directly relevant to spectral interpretation. The subjects are divided into two major sections; the first section provides extensive coverage of each of the individual methods, and the second emphasizes the way these strategies are blended together in the solution of problems based on combined spectra. ... Read more

Reviews (1)

Organic Spectroscopy Made Clear
Crews is not a hardcore treatise of the subject. It does not cover the quantum mechanics behind spectroscopy and the complicated mathematical underneath the concept. Instead it discusses the fundamentals and results of such quantum calculations which apply to identification of organic compounds. Basic concepts of nuclear magnetic resonance, infrared spectroscopy, ultra violet spectroscopy are thoroughly covered with examples and charts. Crews is a splendidly-written, straight-forward work that would be suitable for advanced undergraduates who had finished at least one year of organic chemistry, for those who pursue chemical research, and entering grad students. ... Read more

Book Description

In response to the expansion of knowledge in biochemistry and molecular biology, the Second Edition of this reference has been completely revised and updated, with approximately 16,000 new entries. Names of specific compounds and other substances have been substantially enlarged, and definitions have been expanded for clarity and precision. Information is drawn from over 500 books and 1,000 articles, including recommendations of the Commission on Biochemical Nomenclature, the International Union of Pure and Applied Chemistry, and the International Union of Biochemistry. Terms used by biochemists from a broad range of sciences, such as chemistry, immunology, genetics, virology, biophysics, and microbiology, are included. Abbreviations, both standard and nonstandard, are also provided, as well as cross-referenced synonymous expressions. ... Read more

Reviews (1)

A biochemistry dictionary for Brazilian people
Well, being a Brazilian teacher, I expect to find real good books for us... As you all know, we here do not have much to expend with literature... Our researches are singular, because we really try to do our best. This book brings a brightful help for the biochemical community. Its language is accessible, easy-reading, the examples are usual and modern, slightly different from the ones we were used to. I hope that it should help other biochemistry teachers, and not only in undeveloped Countries. My best wishes, Prof. Raquel Nogueira Lima ... Read more

Book Description

Fully updated and expanded-a solid foundation for understanding experimental enzymology.
This practical, up-to-date survey is designed for a broad spectrum of biological and chemical scientists who are beginning to delve into modern enzymology. Enzymes, Second Edition explains the structural complexities of proteins and enzymes and the mechanisms by which enzymes perform their catalytic functions. The book provides illustrative examples from the contemporary literature to guide the reader through concepts and data analysis procedures. Clear, well-written descriptions simplify the complex mathematical treatment of enzyme kinetic data, and numerous citations at the end of each chapter enable the reader to access the primary literature and more in-depth treatments of specific topics.
This Second Edition of Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis features refined and expanded coverage of many concepts, while retaining the introductory nature of the book. Important new features include:
* A new chapter on protein-ligand binding equilibria
* Expanded coverage of chemical mechanisms in enzyme catalysis and experimental measurements of enzyme activity
* Updated and refined discussions of enzyme inhibitors and multiple substrate reactions
* Coverage of current practical applications to the study of enzymology
Supplemented with appendices providing contact information for suppliers of reagents and equipment for enzyme studies, as well as a survey of useful Internet sites and computer software for enzymatic data analysis, Enzymes, Second Edition is the ultimate practical guide for scientists and students in biochemical, pharmaceutical, biotechnical, medicinal, and agricultural/food-related research. ... Read more

Reviews (3)

A very useful Guide to Enzymes
This book is written by an enzymologist who presented a course on enzymology at a pharmaceutical company, as well as at the University of Pennsylvania. It is a rather well- written book that covers both theoretical and practical aspects of enzyme studies. Black and white drawings and numerous graphs illustrate the concepts. The book begins with a general review of some chemical concepts, including a brief review of thermodynamics, transition states in chemical reactions, acid-base concepts, non-covalent interactions and rates of chemical reactions. The next chapter covers aspects of enzyme (protein) structure. In a departure from other texts, the 4th chapter introduces and develops the concepts of protein-ligand binding equilibria. The derivation of Kd, along with the Langmuir isotherm to derive measurements at equilibrium is developed, as is treatment of equilibrium ligand binding data. The detour into non-enzymatic ligand-protein interactions is a very welcome treatment of this topic, so important in many drug interactions with receptors. While not strictly speaking enzymology, this chapter serves to introduce concepts that are further developed in the next chapter, which deals with the kinetics of enzymatic catalysis. This chapter nicely develops the basic equations and treatment of enzyme kinetics, and the steady state model is developed using the treatment of Briggs and Haldane. A discussion of the significance of both Km and Kcat is followed by methods to derive these values experimentally. After covering kinetics, the book moves on to chemical mechanisms of enzyme catalysis. The importance of transition state stabilization is discussed, and covalent catalysis and acid-base catalysis are highlighted. Serine proteases as model enzyme mechanisms round out the chapter.

Chapters 7 and 8 cover practical aspects of enzymology, such as velocity measurements, continuous versus end point reactions, detection methods for assays, and separation methods for reaction products (e.g., HPLC, TLC). Some pointers on enzyme concentration, temperature, pH and buffer effects on enzyme assays are valuable. The next chapter deals with reversible inhibitors, and describes the derivation of Ki. The distinctions among competitive, non-competitive and uncompetitive inhibition are discussed, and details on how to determine these in a practical sense are illustrated. There is also some discussion around the SAR of inhibitors, and this is tied in with inhibitor and drug design. Tight binding inhibitors get their own brief chapter, and there is a separate chapter on time dependent inhibition and the methodology around measuring this phenomenon. Enzyme reactions with multiple substrates and cooperativity in enzymatic catalysis also receive individual chapter treatments. Two appendices on suppliers and software tools round out the text.

Viva la Sequel!!
I never thought this could happen to me. I picked up the book and I was hooked instantly. Never before in my forays into biochemistry have I been this enchanted by such delightful prose. I started in a thermodynamic sink and I ended as an ultra-high energy intermediate. Copeland added a section on equilibrium binding that bound me with delight. His inhibitor sections loosened all my inhibitions. Even a chowder slurping ingrate from Harvard could understand and appreciate this work.

A real page-turner
In this gripping work, professor Copeland takes the reader on a roller-coaster ride through the thrilling activation energy peaks and local free energy minima of enzymology. From friendly Michealis-Menten kinetics to frightening multi-subtrate systems, Copeland holds the readers hand while unveiling his bold vision of the enzymatic universe. Even a Yalie could understand this post-modern classic. ... Read more

Book Description

Gives medical students 500 questions, answers, and explanations to prepare for the biochemistry and genetics sections of the USMLE Step 1. The new edition includes many new questions in the two-step clinical format to simulate the USMLE Step 1. ... Read more

Reviews (2)

Great question & review book
I found this book very helpful in my step 1 prepertion most of all helped me with Molecular biology questions in my actual test and is also great for genetics, over all the more questions you do from this book the more it boosts your confidence in Biochemistry in the real exam specially if you were like me (an IMG)whose Biochemistry background is weak and feel like practicing more questions, you will definitely make use of this book

helpful and good assistent in your study
it is easy to use and is giving the most popular type of question you need to pass USMLE step I so I recommended it for everybody who wants to pass the step with high score as I am doing . ... Read more

Book Description

A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing.
Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use.

Key Features
* Provides general guidelines and strategies for isolation of membrane proteins
* Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs
* Gives special emphasis to new native and denaturing electrophoresis techniques
* Explains modifications of techniques used for water-soluble proteins ... Read more

Reviews (1)

A simple and much enough contents for everyone
Membrane proteins have many typical characters that are very different from other water-soluble proteins. You can learn almost about topics of the membrane protein simply. This book is constructed with two big chapters and first chapter gives explanations about general strategies and techniques about purification (choice of protein sources, solubilization techniques, etc.), chromatography (hydroxylapatite, ion-exchange, chromatofocusing, metal chelate, affinity, covalent, hydrophobic, gel filtration, etc.) and electrophoresis(denaturing, native gel). In second chapter, several examples of membrane proteins were reported. Each purification procedures were detailed and you will be able to understand step by step. This book covers totally about practical membrane protein science and will be a nice guide for beginners as well as experts. ... Read more

Reviews (2)

Good if you need quick quick coverage.
Nicely summed up by the reviewer below. If you need a very quick-and-dirty review, this is good. I take points off due to the lack of index and some weak sections. At this point, QBank leads me to believe that this book may not cover everything I needed to know.. but good for last minute stuff, especially if you hate Biochem like myself.

Good choice for USMLE
High Yield Biochemistry is the perfect book for a one or two day board review. It is easy reading without the complex charts and detail needed in the first year of medical school. The book does miss some major points. Vitamin overdoses are weak, as are some major disorders/deficiencies like Diabetes, G6pdh, Hbs/Hbc, and purine salvage. ... Read more

Book Description

Designed to supplement and complement any standard biochemistry text or lecture notes, this book helps provide a balanced picture of modern biochemistry by use of elementary mathematics in understanding properties and behavior of biological molecules. It provides a balanced picture of modern biochemistry by using elementary mathematics to explore the properties and behavior of biological molecules. The text discusses such topics as:

Book Description

As the first comprehensive overview of the nucleolus since 1985, The Nucleolus covers our current understanding of the cell nucleolus, including its role in ribosome assembly and its additional newly-discovered activities. The eighteen chapters have been written by experts who are actively engaged in research on the nucleolus and have an in depth review of the following topics: nucleolar ultrastructure and dynamics, behavior during mitosis, ribosomal DNA gene and chromatin structure, pre-ribosomal RNA transcription, processing and modification, ribosome assembly, small nucleolar RNAs, proteomics and non-traditional functions of the nucleolus. ú/LISTú Separate chapters are also provided for yeast and higher eukaryotes on many topics.
The Nucleolus will appeal not only to scientists directly engaged in nucleolar research, but also those working in related areas such as gene expression, protein biosynthesis, ribosome structure, transcription, chromatin structure, molecular genetics and the structure and functions of the cell nucleus in general. ... Read more

Book Description

Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book will be a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians. ... Read more

Reviews (1)

A brilliantly explained text
A brilliantly explained text containing extremely good applications, and above all, clear and well-referenced recipes. Most pitfalls in microscopy are the result of not following optimal processing conditions. After reading Dr. Hayat's present publication, there is no excuse for not following well-tested reliable protocols.

This Handbook is the most authoritative publication in the disciplines of microscopy, immunohistochemistry and antigen retrieval methods. It is expected to become a classic in teaching and research. This book should not be absent in your personal collection, as well at the library.

- Taken from a review by Prof. Dr. E. Marani, Afdeling Fysiologie, Leiden, The Netherlands. ... Read more

Book Description

Most medicinal chemists working in the pharmaceutical industry are organic synthetic chemists with little or no background in medicinal chemistry.These chemists must acquire a good knowledge of medicinal chemistry during their firstyears in the pharmaceutical/drug discovery industry. This bookaims to be their practical handbook - a complete guide to the drug discovery process.

The book reviews practical aspects of Medicinal Chemistry, emphasising the daily problems met by the medicinal chemist when dealing with lead discovery/identification methodologies, with structure-activity relationship studies aimed to scale up potency and target selectivity, and when optimising pharmacokinetic and pharmaceutical properties by means of ultimate chemical modifications.

This is the second edition of The Practice of Medicinal Chemistry, the first edition being published in February 1996.The new edition is thoroughly revised, with around 30% new material, and refocussed to reflect the recent developments in genomics, proteomics, high throughput screening of compounds and drug solubilisation.An effort has been made to remove the overlap that exists in the previous edition and four new chapters have been introduced.

* Written by experts in the field
* The first edition of The Practice of Medicinal Chemistry, published in 1996, was nicknamed 'The Bible' by medicinal chemists.
* The only available book dealing with the practical aspects of medicinal chemistry, from conception of the molecules through to the production of drugs
* An essential practical handbook for the medicinal chemist, working in the pharmaceutical industry, withlittle or no background in medicinal chemistry-a complete guide to the drug discovery process. ... Read more

Reviews (1)

Pls help me to get this book.
I want to buy this book. I am in Japan. Would you pls tell me how I can get this book. ... Read more

Reviews (1)

An informative introductory text.
Overall, this book is a useful reference for anyone engaging in a Bioinorganic course at the upper undergraduate or early postgraduate level. This book is sufficiently informative and can be used as a recommemded text for the upper undergraduate level in a course in Bioinorganic Chemistry. ... Read more

Book Description

The latest edition of this highly acclaimed textbook, provides a comprehensive and up-to-date overview of the science and medical applications of biopharmaceutical products. Biopharmaceuticals refers to pharmaceutical substances derived from biological sources, and increasingly, it is synonymous with 'newer' pharmaceutical substances derived from genetic engineering or hybridoma technology.

This superbly written review of the important areas of investigation in the field, covers drug production, plus the biochemical and molecular mechanisms of action together with the biotechnology of major biopharmaceutical types on the market or currently under development. There is also additional material reflecting both the technical advances in the area and detailed information on key topics such as the influence of genomics on drug discovery. ... Read more

Book Description

John R. Crowther provides today's premier practical guide to the understanding and application of ELISA. Updating and greatly expanding his widely appreciated earlier publication, ELISA Theory and Practice (1995), this important work introduces chapters on such major new topics as checkerboard titrations, quality control of testing, kit production and control, novel monoclonal antibodies, validation of assays, statistical requirements for data examination, and epidemiological considerations. With its numerous worked examples, detailed instructions, and extensive illustrations, The ELISA Guidebook offers a powerful synthesis of all the basic concepts and practical experimental details investigators need to understand, develop, and apply the new ELISA methodology successfully in day-to-day basic and clinical research. ... Read more

Reviews (7)

A Cleverly Designed Book
Some biochemistry books proceed to describe metabolic pathways with scant reference to the physiological context in which they occur and then fill the conceptual gap by including a chapter on regulation and integration of metabolism. Not this thoughtful and cleverly designed book. From the start the authors make it clear that they are going to tell us the story in terms of one crucial unifying concept: which pathways are activated or inactivated when we eat or when we fast?

With this approach the reader never loses sight of the big picture, ie, what's going on in the body at any particular time. And then, when we do reach the chapter on integration of metabolism, most of the work has already been done for us: the chapter then operates as a useful review of what we have already been told.

The book does not miss any opportunity to make us think in terms of the big picture, and furthermore, it is never afraid of saying something more than once if it needs to. Just one example to illustrate this point: Lipoprotein lipase. Action and properties of this enzyme are detailed in the context of digestion of chylomicrons (p 497), storage of TAGs in adipocytes (p 512), integration of metabolism (p 561) and the influence of insulin levels on gene expression of the enzyme (p 564).

The early introduction of the fed and starved states is also useful in other ways. The student perceives right away what the point is of learning those endless metabolic pathways and studies them with much more enthusiasm and not just thinking that biochemistry is but an unnecessary obstacle they have to overcome to get to the more clinical stuff (at least I'd like to think so!). Inspired by this book I have organized my course in that way and I am convinced that it is the best approach.

Simple, Lucid, yet encylopedic
This book is different from its contemporaries in many ways. First of all is written in a clinical approach format which is very interesting for medical students. Most of the biochemistry textbooks are dry and vast and difficult to grasp the information needed for a medico.
But the Marks helps to learn to use biochemistry in the process of Clinical Problem Solving. With a lot of diagrams and clear text it is easy understand.
The Clinical Material is presented in the form of real patients.
It is a student friendly text with which you can learn biochemistry in a funny way of solving the clinical cases.
It can be used as a main textbook for self study.
I strongly recommend the Marks for all medical students.

Marks Biochemistry
This books is great.If you want to leran biochemistry within a month,buy it.I think it is the best book.It is perfect for your exam.

Not Pretty Good for USMLE
Texts are not easily to follow. Diagrams and help notes scatters around pages which make readers confused. If you are preparing for Step 1, I recommend you study Lippincott Biochemistry, Board Review Series.

This is the best biochemistry book (easy and comprehensive)
I love this book on biochemistry simply because it was the easiest to read, with diagrams and pictures even dumb people like me can read (gg). I also found that it is very comprehensive also. Some of the other titles I've read on the subject (Lippincott, Harper's) did not go into the detail of this one. Another positive note is that this is a clinical book also; giving you case studies in each chapter to test your knowledge. I can't say enough about this book and even referred it to one of my friends. Although not required in Chiropractic College (Lippincott was), it should have been the standard. ... Read more

Book Description

Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein–protein interactions and networks. ... Read more

Reviews (2)

Very readable introduction to Proteomics
This very readable book provides an excellent overview of proteomics. It gives a survey of techniques, comparing them and explaining why and when they are used. This is not the place to learn any method in detail. But it is the only book I have seen that you can read cover to cover in a few days and get the big picture of the field. Some caveats: each chapter has only 3 or 4 references, and the book focuses on methods connected to mass spectrometry. But if you want a quick and pleasant introduction to methods for purifying and identifying proteins, including discussions of ICAT, protein complexes and post-translation modifications, I highly recommend this book.

Good information, poor organization
There really are lots of good facts in this book. Somehow, though, they never come together to form a coherent whole.

Most chapters read like essays, meant to stand on their own. That means that many chapters repeat introductory material already covered, and remake points already made. Yes, I appreciate that silver staining does not properly represent the whole range of protein expression levels. I do not appreciate the need to stat that three or four times.

Despite its "Introduction" title, the author seems to forget what a novice will need to learn. For example, there's a discussion of isoelectric focussing for protein separation. That includes mention of the technique's history, and why it used to have reproducibility problems. I never did see an exact description of what the techique actually is or how it works, though. Likewise, the author describes a number of important software packages used in proteomics. There are no screen shots, though, comparisons of capabilities, details of usage, or even references for letting me find out more on my own.

When I got to the the end of this book, I wanted to go back and review a few points. Because of the generally poor organization, it was always a struggle to figure out just which discussion covered the topics I wanted.

Other books cover almost all of this title's content, and do so more clearly. I suggest that the interested reader keep on looking. ... Read more

Book Description

Where do the roots of addictive behavior lie -- in our genes or in our environment, in our chemistry or in our character? In the Craving Brain, Dr. Ronald Ruden asserts that the roots of addiction most defintetly do not lie in our character. Rather, they lie in a complex chain reaction that originates in an ancient survival mechanism in the brain. When this system is inappropriately activated, it drives the body to crave, sometimes with addictive behavior as the end result. In clear, straightforward language, Dr. Ruden outlines his remarkable successful treatment program which he believes can cure this problem.

The Craving Brain offers crucial insights into the world of addiction. This revolutionary book will bring hope to millions of people who suffer from a wide range of addictions, from gambling and alcohol to drugs and food.

Reviews (3)

Understanding Craving and Addiction
This is a great little book for both the lay public and professionals. I highly recommend it. It provides a good, solid scientific understanding of addiction in simple language and offers the reader useful guidelines about moving beyond sobriety and toward cure. --Fred P. Gallo, Ph.D., Author of "Energy Psychology" and "Energy Tapping."

i was addicted to the information
This book is very informative. It is a great tool for those who need to understand themselves.

Must read book for addiction professionals
This is an excellent and cutting edge explanation of addiction that ties together not only chemical dependencies but process dependencies. Also a very hopeful book in that answers are presented and current approaches validated and explained as to why they are working for those who apply them. Give it a read! ... Read more