Book Description

The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to:
* Optimize novel applications
* Avoid many cumbersome molecular biological techniques
* Set up the laboratory to avoid contamination

Key Features
* This first-rate guide will help you
* Avoid contamination--with specific instructions on setting up your lab
* Avoid cumbersome molecular biological techniques
* Discover new applications
* Simply call our toll-free, 800 number listed below or cut out the coupon, fill in the blanks, and mail it to us with your check, credit-card number, or money order; We'll send you the book and you'll get your answers ... Read more

Book Description

The first comprehensive professional book of its kind, this combination text and atlas features the most up-to-date information on the evaluation of adult and adolescent sexual assault victims. It can serve as a complete reference manual. ... Read more

Reviews (1)

Excellent resource for S.A.N.E.s!
This is a MUST have for any nurse practicing as a S.A.N.E. (Sexual Assault Nurse Examiner). Crowley's information is on the mark and written in an informative, very appropriate language. A handy reference while completing an exam or preparing for the next to come. ... Read more

Book Description

Immunoassays are among the most powerful and sensitive technologies now available for patient diagnosis and monitoring. This book is an indispensable guide to information on the theory and practice of immunoassays. It discusses the scientific basis of these technologies in a logical, organized, and heuristic manner and provides protocols for specific assays. The contents of this unique book are balanced among theory, practical issues, quality control, automation, and subspecialty areas, making it ideal for health science students, laboratory scientists, and clinicians.

Key Features
* Presents up-to-date information
* Provides extensive cross-referencing
* Covers theory and practice in full detail
* Written by leading authorities ... Read more

Book Description

This practical guide provides information essential to understanding the significance of molecular biology and its application to everyday practice. Coverage focuses on the spectrum of currently used molecular tests in the areas of hematopathology, solid tumors, genetics, and identify testing. Examples of both molecular and cytogenetic assays demonstrate how these tests are used in today's pathology labs to detect a wide range of infectious diseases and inherited and metabolic conditions. ... Read more

Book Description

Presenting the newly revised and updated edition of Fletcher's renowned Diagnostic Histopathology of Tumors . This extensively referenced 2-volume work, is unique in its coverage of the subject, providing comprehensive information on the vast range of tumor types and most up-to-date biopsy methods currently used in pathology practice today.An abundance of new excellent-quality color photos have been added to help illustrate important morphologic findings and relate these to their specific classifications. ... Read more

Reviews (2)

The best book of tumoral histopathology in this time!!!!
well, my english is not so good, but I'll try to explain my opinions about this extraordinary book: I'm a proffesor of Pathology in Maracaibo, Venezuela, especifically in Veterinary Pathology and mi field of studied is the comparative pathology of tumours. This book represented the most advanced updated that I have been read. The ilustrations and the text are of high quality, very concise and clear. I recommended this excellent book both veterinary pathologist and human pathologist

Diagnostic Histopathology of Tumours
An excellent reference book for postgraduates and practitioners of Anatomical Pathology. ... Read more

Book Description

Significant advances in DNA analysis techniques have surfaced since the 1997 publication of the bestselling An Introduction to Forensic DNA Analysis. DNA typing has become increasingly automated and miniaturized. Also, with the advent of Short Tandem Repeat (STR) technology, even the most minute sample of degraded DNA can yield a profile, providing valuable case information. However, just as the judicial system slowly and reluctantly accepted RFLP and AmpliType® PM+DQA1 typing, it is now scrutinizing the admissibility of STRs.Acknowledging STR typing as the current system of choice, An Introduction to Forensic DNA Analysis, Second Edition translates new and established concepts into plain English so that laypeople can gain insight into how DNA analysis works, from sample collection to interpretation of results. In response to the shift toward more efficient techniques, the authors cover the legal admissibility of STR typing, expand the chapter on DNA databases, and revise the section on automated analysis. They also present key decisions and appellate or supreme court rulings that provide precedent at the state and federal levels.Discussing forensic DNA issues from both a scientific and a legal perspective, the authors of An Introduction to Forensic DNA Analysis, Second Edition present the material in a manner understandable by professionals in the legal system, law enforcement, and forensic science. They cover general principles in a clear fashion and include a glossary of terms and other useful appendices for easy reference. ... Read more

Reviews (6)

A great intro for the beginner
Having left behind my interest in genetics in order to pursue tax accountancy, I've always been fascinated by accounts of DNA typing. This book provides a wonderful introduction that is accessible to even the novice geneticist like myself. Much of the information Rudin and Inman give is quite practical; with some help from a friend who works in a medical lab I was able to set up my own electrophoresis gels and PCR. A word of caution to the amateur, however: make sure you practice before drawing any conclusions from the "evidence." My wife, Amy, wouldn't speak to me for a week after I claimed that a stain on our bed linen did not match my DNA. It turned out that she had spilled some ice cream.

vivid introduction to forensic genotyping techniques
Nice figures, photos, schemes, and U.S. tables; lively comparisons show that authors are good teachers. Though technical part of the book outdates fast necessarily (acronym SNP has not been coined in 1996 yet), it is a shame that an interpretation part of the book neglects the current knowledge. Bayes theorem is known since 1763 and, nevertheless, likelihood ratio is not even mentined in the book! Books of Evett and Weir or of Robertson and Vignaux cannot be substituted by this book.

A must-have for any DNA criminalist or criminal lawyer
I have a BS in genetics and biochemistry and am looking to enter into a forensics lab. This book is an EXCELLENT resource for an entry-level criminalist, criminal lawyer, or the non-scientist interested in this topic. It was organized from basic genetics to higher-level interpretation issues and included tons of diagrams, pictures, and relevant case studies. This book did an outstanding job explaining complicated and detailed subject matter in an easy to understand and interesting matter.

Attorney's Guide
As a practicing lawyer who earns a living in the criminal courts, I found this book an excellent and informative guide to this often problematic area. The authors introduce the subject in a way that is easy for the beginner to comprehend, but at the same time include sufficient detail to answer many of the most pressing problems facing a defence team in court.

This showed an apt knowledge of DNA and it's life applicatio
This was an informative and comprehensive rewiew of typically boring material. I felt a new understanding of the topic of DNA. ... Read more

Book Description

Blueprints Clinical Procedures combines almost 300 color photos with clear, step-by-step instructions for nearly 100 commonly performed procedures. Finally, instead of lengthy searches through multiple sources, you’ll be able to find ALL the procedures you need to know, collected into one handy resource.

Blueprints Clinical Procedures features:

Color photos illustrate how to perform procedures – perfect for a quick "see one" review before you have to "do one."

Pearls/Tips boxes containing critical information such as contraindications, and special "how-to" advice, similar to the information received from residents and attendings while on rounds

Special How to Interpret section explains interpretation of ABGs, chest, and abdominal x-rays…a vital addition to clinical work

Valuable Appendices on:
Writing progress and procedure notes, and "SOAP" notes
Taking a complete history
Spanish/English History and Physical Terms and Phrases

Sterile glove icon next to the title of procedures performed under sterile conditions – reminding you to prepare properly before you begin.

Whether performing a procedure for the very first time, or polishing your technique, Blueprints Clinical Procedures provides the vital content you need to be accurate and safe. A must-have for all health care professionals who perform procedures and an invaluable resource to use while in school and later in residency. ... Read more

Book Description

From its first-published account in 1985, the polymerase chain reaction has become a standard research tool in a wide range of laboratories. Its impact has been felt in basic molecular biological research, clinical research, forensics, evolutionary studies, and the Human Genome Project. The PCR technique originally conceived by Nobel laureate Kary Mullis has proven to be exceptionally adaptable and has been transformed into a myriad array of methods, each with different applications.

PCR Primer: A Laboratory Manual introduces the complex world of PCR by beginning at an accessible level and then moving to more advanced levels of application. First, the practical requirements for performing PCR and other amplification techniques in the lab are introduced and then the basic aspects of the technique are explained by exploring important issues such as sample preparation, primer design, efficiency, detection of products, and quantitation. Protocols for a wide range of PCR and amplification techniques—each written by an expert investigator—are presented for cloning, sequencing, mutagenesis, footprinting, library construction and screening, exon trapping, differential display, and expression, and these include RT-PCR, RNA PCR, LCR, multiplex PCR, panhandle PCR, capture PCR, expression PCR, 3' and 5' RACE, immune PCR, in situ PCR, and ligation-mediated PCR. Each protocol is augmented by analysis and troubleshooting sections and complete references. ... Read more

Reviews (1)

Best PCR manual there is!
I have used two different protocols from this book, 1, RACE-PCR and 2, mutagenesis.These two methods were described very well, both theoretically and practically. The protocols were described in detail, were easy to follow, and they work! I'm sure that the other protocols in this book also work equally well. If you do PCR, you need this book! ... Read more

Book Description

Since the publication of the second edition of Free Radicals in Biology and Medicine in 1989, the techniques of modern molecular, cellular, and structural biology have provided new data that have transformed scientific thinking about topics within free radical research. New reactive species, such as peroxynitrite, have been discovered to have a role in biological systems. Much of this third edition has been completely re-written and what remains from the second has been thoroughly updated. There are new chapters on antioxidant defence systems in bacteria, plants, and animals; the detection of free radicals; the role of antioxidants in aging, nutrition, disease, and therapy; and a new appendix on molecular biology. The chapter on oxidative stress and its consequences includes the damage and repair of DNA, proteins, and lipids, cytokines, and cell death (both apoptosis and necrosis).

Free radicals and other reactive species are now implicated in a wide range and number of diseases and the third edition covers their role in hypertension, diabetes, lung damage, cystic fibrosis, nervous system disorders, viral infections, and cancer. The toxicology chapter has been updated to include new sections on complex mixtures, nitro- and azo-compounds, and radiation. Erythrocytes, chloroplasts, the eye, the skin, reproduction, and exercise are covered as special cases of oxidative stress and antioxidant protection. Reactive species can act as useful biomolecules and this role is fully discussed. There is also an up-to-date introduction to free radical chemistry (as well as a basic chemistry appendix) and oxygen toxicity. Each major statement in the text is referenced. The authors have maintained the simplicity of approach and readability of the previous editions to make this book an invaluable companion to all those interested in the role of free radicals in life. ... Read more

Reviews (2)

Notes from an Oxymoron
Excellent book that provides a thorough grounding to the field. Keep the updated editions coming every few years and it will continue to stay at the top.

An excellent book for scientists
Free radical biology has become a big research area. This text is definitely a helpful tool for students and professionals alike. Halliwell and Gutteridge are both the "authorities" in free radical research. The book contains the basic concepts of metabolism, metal interactions, free radical formation, and antioxidation. The book then relates these processes (that is, oxidative and nitorsative stresses) to pathological events using various disease paradigms. The text contains good illustrations. It is an excellent reference material for everyone who is entering the field of free radical biology and medicine. ... Read more