Book Description

This book covers both basic elements and the state-of-the-art in applications of proteomics. The first section gives an introduction to the field, from differential approaches to functional proteomics, including yeast two-hybrid, mass spectrometry, mapping of post-translational modifications, and NMR spectroscopy. The second section offers a complete picture of industry studies in proteomics for human health and biotechnology applications. The final section addresses the future, looking at the most promising areas such as protein arrays and the integration of genomic and proteomic tools. ... Read more

Book Description

A unique exploration of the principles and methods underlying the Human Genome Project and modern molecular genetics and biotechnology-from two top researchers

In Genomics, Charles R. Cantor, former director of the Human Genome Project, and Cassandra L. Smith give the first integral overview of the strategies and technologies behind the Human Genome Project and the field of molecular genetics and biotechnology. Written with a range of readers in mind-from chemists and biologists to computer scientists and engineers-the book begins with a review of the basic properties of DNA and the chromosomes that package it in cells. The authors describe the three main techniques used in DNA analysis-hybridization, polymerase chain reaction, and electrophoresis-and present a complete exploration of DNA mapping in its many different forms. By explaining both the theoretical principles and practical foundations of modern molecular genetics to a wide audience, the book brings the scientific community closer to the ultimate goal of understanding the biological function of DNA. Genomics features:

• Topical organization within chapters for easy reference
• A discussion of the developing methods of sequencing, such as sequencing by hybridization (SBH) in which data is read through words instead of letters
• Detailed explanations and critical evaluations of the many different types of DNA maps that can be generated-including cytogenic and restriction maps as well as interspecies cell hybrids
• Informed predictions for the future of DNA sequencing

Reviews (2)

Genomics
So far I heard that this is the one of the best book containing all informations and instructions about genome project. I love to review this book throgh e-mail.
I can't afford myself to buy this book. but so far i can say if it is really very much helpful to me i recommend evybody of my class (MSC Bioinformatics) to go for buying that book.

with thanks

a 'Gene VI' in Postgenome era
as good as GENE VI by Lewis ... Read more

Book Description

This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book. It also provides detailed protocols as well as many helpful hints to achieve experimental success and to avoid pitfalls. ... Read more

Book Description

As both an informational molecule and a catalyst, RNA may hold clues to the emergence of genetic self-replication and the origins of life. The first, 1993 edition of this important work was acclaimed as unique and authoritative. The new edition has been revised, updated, and extended, and offers a completely current perspective on the modern world of RNA and the light it sheds on a prebiotic era perhaps dominated by this extraordinarily versatile molecule. This book is essential reading for everyone interested in the biology of nucleic acids, and is a valuable resource for teaching as well as investigative science. ... Read more

Reviews (1)

Excellent read.
As a second edition, this book is not just an updated version of the original. In fact, it should probably have been called volume 2, instead of second edition. If you have the original, you will want to put this on the shelf next to it. The book covers a great deal in a field that is rapidly moving forward. A must for people interested in what is going on in "the RNA world." ... Read more

Book Description

Fully updated and expanded-a solid foundation for understanding experimental enzymology.
This practical, up-to-date survey is designed for a broad spectrum of biological and chemical scientists who are beginning to delve into modern enzymology. Enzymes, Second Edition explains the structural complexities of proteins and enzymes and the mechanisms by which enzymes perform their catalytic functions. The book provides illustrative examples from the contemporary literature to guide the reader through concepts and data analysis procedures. Clear, well-written descriptions simplify the complex mathematical treatment of enzyme kinetic data, and numerous citations at the end of each chapter enable the reader to access the primary literature and more in-depth treatments of specific topics.
This Second Edition of Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis features refined and expanded coverage of many concepts, while retaining the introductory nature of the book. Important new features include:
* A new chapter on protein-ligand binding equilibria
* Expanded coverage of chemical mechanisms in enzyme catalysis and experimental measurements of enzyme activity
* Updated and refined discussions of enzyme inhibitors and multiple substrate reactions
* Coverage of current practical applications to the study of enzymology
Supplemented with appendices providing contact information for suppliers of reagents and equipment for enzyme studies, as well as a survey of useful Internet sites and computer software for enzymatic data analysis, Enzymes, Second Edition is the ultimate practical guide for scientists and students in biochemical, pharmaceutical, biotechnical, medicinal, and agricultural/food-related research. ... Read more

Reviews (3)

A very useful Guide to Enzymes
This book is written by an enzymologist who presented a course on enzymology at a pharmaceutical company, as well as at the University of Pennsylvania. It is a rather well- written book that covers both theoretical and practical aspects of enzyme studies. Black and white drawings and numerous graphs illustrate the concepts. The book begins with a general review of some chemical concepts, including a brief review of thermodynamics, transition states in chemical reactions, acid-base concepts, non-covalent interactions and rates of chemical reactions. The next chapter covers aspects of enzyme (protein) structure. In a departure from other texts, the 4th chapter introduces and develops the concepts of protein-ligand binding equilibria. The derivation of Kd, along with the Langmuir isotherm to derive measurements at equilibrium is developed, as is treatment of equilibrium ligand binding data. The detour into non-enzymatic ligand-protein interactions is a very welcome treatment of this topic, so important in many drug interactions with receptors. While not strictly speaking enzymology, this chapter serves to introduce concepts that are further developed in the next chapter, which deals with the kinetics of enzymatic catalysis. This chapter nicely develops the basic equations and treatment of enzyme kinetics, and the steady state model is developed using the treatment of Briggs and Haldane. A discussion of the significance of both Km and Kcat is followed by methods to derive these values experimentally. After covering kinetics, the book moves on to chemical mechanisms of enzyme catalysis. The importance of transition state stabilization is discussed, and covalent catalysis and acid-base catalysis are highlighted. Serine proteases as model enzyme mechanisms round out the chapter.

Chapters 7 and 8 cover practical aspects of enzymology, such as velocity measurements, continuous versus end point reactions, detection methods for assays, and separation methods for reaction products (e.g., HPLC, TLC). Some pointers on enzyme concentration, temperature, pH and buffer effects on enzyme assays are valuable. The next chapter deals with reversible inhibitors, and describes the derivation of Ki. The distinctions among competitive, non-competitive and uncompetitive inhibition are discussed, and details on how to determine these in a practical sense are illustrated. There is also some discussion around the SAR of inhibitors, and this is tied in with inhibitor and drug design. Tight binding inhibitors get their own brief chapter, and there is a separate chapter on time dependent inhibition and the methodology around measuring this phenomenon. Enzyme reactions with multiple substrates and cooperativity in enzymatic catalysis also receive individual chapter treatments. Two appendices on suppliers and software tools round out the text.

Viva la Sequel!!
I never thought this could happen to me. I picked up the book and I was hooked instantly. Never before in my forays into biochemistry have I been this enchanted by such delightful prose. I started in a thermodynamic sink and I ended as an ultra-high energy intermediate. Copeland added a section on equilibrium binding that bound me with delight. His inhibitor sections loosened all my inhibitions. Even a chowder slurping ingrate from Harvard could understand and appreciate this work.

A real page-turner
In this gripping work, professor Copeland takes the reader on a roller-coaster ride through the thrilling activation energy peaks and local free energy minima of enzymology. From friendly Michealis-Menten kinetics to frightening multi-subtrate systems, Copeland holds the readers hand while unveiling his bold vision of the enzymatic universe. Even a Yalie could understand this post-modern classic. ... Read more

Book Description

In this first book to provide an overview of applications of proteomics in the discovery of new diagnostic, prognostic and therapeutic targets, a team of international specialists from research institutions, hospitals and companies contribute with their specific expertise. They cover a wide range of example applications for the most important diseases, such as heart and cardiovascular disorders, cancer, pharmatoxicology, infectious diseases and diseases of the nervous system.
Denis Hochstrasser is an eminent scientist in the field of bioinformatics and proteomics and one of the founders of the Swiss Prot Databank as well as of the Swiss biotech company Genebio.

... Read more

Book Description

Arguably no other field of biological research embraces such a diverse array of experimental approaches as does the field of calcium signaling. Not only does itspan virtually all conceptual and technical areas of molecular and cell biology, but a number of unique techniques, such as the use of permeabilized cells to study intracellular calcium metabolism, have evolved directly from calcium research.Calcium Signaling explores this fascinating area of research, covering the biophysical, molecular biological, and cell biological techniques involved in the study of calcium signaling. This work examines a broad range of topics-from techniques for imaging and measuring calcium in subcellular compartments, to subcellular fractionation and intracellular calcium stores, and patch clamp investigation of calcium channels.Filled with detailed illustrations, Calcium Signaling addresses those key methodological approaches that are useful to scientists and researchers investigating calcium function and regulation in many diverse areas of biological and medical research. ... Read more

Book Description

Halogenated organic compounds constitute one of the largestgroups of environmental chemicals. The industrial production of newhalogenated organic compounds has increased throughout the lastcentury and these compounds are integral to a variety of industrialapplications. Although organohalide compounds are typically consideredto be anthropogenic industrial compounds, these have their counterpartin several thousands of natural biogenic and geogenic organohalides,representing most classes of organic chemicals. Natural sourcesaccount for a significant portion of the global organohalogen budget.This volume, authored by leading experts in the field, provides acurrent perspective on how both natural and synthetic organohalidesare formed and degraded, and how these processes are incorporated intoa global halogen cycle. The list of organohalides that can be utilizedby microbes continues to increase dramatically, as do the number ofdehalogenating microorganisms that have been identified andcharacterized. A critical step in the degradation of organohalides iscleavage of the carbon-halogen bond, and microorganisms have evolved avariety of metabolic strategies for dehalogenation. The chaptersprovide a global perspective on the diversity of dehalogenatingmicroorganisms, explore their ecology, biochemistry and genetics, andreview the range of biologically-mediated dehalogenation mechanisms.Many of the problematic organohalides, such as pesticides,chlorofluorocarbons, chlorinated solvents, polychlorinateddibenzo-p-dioxins and polychlorinated biphenyls, are covered indetail. The book provides a comprehensive overview of fate of thesecompounds in the environment, practical applications in the laboratoryand the field, and strategies for the development of bioremediationtechnologies for organohalide-contaminated sites. Detailed information on biodegradation and biotransformationmechanisms for a variety of organohalides and on the microorganismsmediating these processes has greatly increased our understanding ofthe cycling and fate of these unique and widespread compounds in ourenvironment. The book will serve as a comprehensive resource on theprocesses and applications of microbial degradation of halogenatedorganic compounds. ... Read more

Book Description

This volume contains the Proceedings of the 8th InternationalSymposium on Magnesium. It presents research and applications in orderto interface between medical doctors, clinicians and scientistsresponsible for magnesium involvement in the pathogenesis of diseases,its biological significance, metabolism and many other utilizationswhich are associated with membranes and cells. The topics which arediscussed concern mechanisms of the mode of action of free magnesiumcations, hydrated cations and magnesium-linked cations. ... Read more

Book Description

A growing body of evidence has begun to reveal flaws in the traditional assumption of female passivity and lack of discrimination after copulation has begun. William Eberhard has compiled an impressive array of research on the ability of females to shape the outcome of mating. He describes studies of many different cryptic mechanisms by which a female can accept a male for copulation but nevertheless reject him as a father. Evidence from various fields indicates that such selectivity by females may be the norm rather than the exception. Because most post-copulatory competition between males for paternity is played out within the bodies of females, female behavior, morphology, and physiology probably often influence male success in these contests. Eberhard draws examples from a diversity of organisms, ranging from ctenophores to scorpions, nematodes to frogs, and crickets to humans.

Cryptic female choice establishes a new bridge between sexual selection theory and reproductive physiology, in particular the physiological effects of male seminal products on female reproductive processes, such as sperm transport, oviposition, and remating. Eberhard interweaves his review of previous studies with speculation on the consequences of this theoretical development, and indicates promising new directions for future research. ... Read more

Book Description

"it is a pleasure just to read this handsome and carefully produced work" Angewandte Chemie 2002

"...the Handbook of Metalloproteins is highly recommended as a resource for bioinorganic chemistry. It will have lasting value for researchers in the field..." The Alchemist - Chemweb

In recent years, the analysis and classification of metalloproteins at the interface between chemistry and biology has accelerated. Many developments and initiatives have taken place and this two-volume handbook provides a comprehensive, yet focussed, collection of 105 major metalloproteins. Content is presented in both a large format and full colour and covers the most relevant transition metals such as Iron, Nickel, Copper, Cobalt, Molybdenum, Manganese Tungsten and Vanadium.

This is the first Handbook of Metalloproteins ever published and is comprised of articles written by renowned experts in the field. It draws together contributions from over two hundred internationally renowned researchers that include: Douglas Rees and Charles Stout as well as Nobel Prize winner Robert Huber.

Each contribution is presented in a similar format and shows a ribbon plot of the overall 3D Structure on their first page, a representation of the metal active site and numerous other figures and tables underpinning the remarks. Comparative information is provided on different proteins and every entry has been extensively referenced to current literature.

• First comprehensive handbook to cover the major metalloproteins
• Presents structural and functional data in an organised manner
• Incorporates full-colour representation of molecular structures throughout
• Unifies information from molecular biology, enzymology, spectroscopy, biochemistry, chemistry, biophysics, macromolecular crystallography and structural biology
• Includes comprehensive sections that cover: Functional Class, Occurrence, Amino Acid Sequence Information, Protein Production, Purification and Molecular Characterisation, Metal Content and Cofactors, Activity Test, Spectroscopy, 3D Structure, Functional Aspects.

Book Description

Protein Physics is a lively presentation of the most general problems of protein structure, folding and function from the physics and chemistry perspective, based on lectures given by the authors. It deals with fibrous, membrane and, most of all, with the best studied water-soluble globular proteins, in both their native and denatured states. The major aspects of protein physics are covered systematically, physico-chemical properties of polypeptide chains; their secondary structures; tertiary structures of proteins and their classification; conformational transitions in protein molecules and their folding; intermediates of protein folding; folding nuclei; physical backgrounds of coding the protein structures by their amino acid sequences and protein functions in relation to the protein structure. The book will be of interest to undergraduate and graduate level students and researchers of biophysics, biochemistry, biology and material science.

* Designed for a wide audience of undergraduate and graduate students, as well as being a reference for researchers in academia and industry
* Covers the most general problems of protein structure, folding, and function and introduces the key concepts and theories
* Deals with fibrous, membrane and especially water-soluble globular proteins, in both their native and denatured states
* Summarizes and presents in a systematic form the results of several decades of world wide fundamental research on protein physics, structure and folding
* Examines experimental data on protein structure in the post-genome era ... Read more

Reviews (2)

Ptitsyn's 'Protein Physics': a book of worth.
A book of worth from the legendary institution, PhysTech (Moscow). Ptitsyn's 'Protein Physics' is a valuable source of information on folding thermodynamics and kinetics both to beginners and professionals in this (and related) fields. No more words. Strongly recommended!

By far the best book on this subject
Excellent book for both beginners and experts. I will recommend it to students as a source of initial knowledge not only on proteins, but also on biopolymers and biophysics in general. For instance, presentation of forces is superb. I am also positive that every expert will find new non trivial insights on great many subtle points of this difficult topic. ... Read more

Book Description

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences. ... Read more

Book Description

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. ... Read more

Book Description

The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done. ... Read more

Book Description

This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there is the best evidence for the structural sophisitication of the protein aggregates.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. ... Read more

Book Description

Proteins: Analysis and Design focuses solely on individual experimental approaches, rather than on specific classes of proteins. The book provides insight into the important issues in protein science and how one can cope with them. These include all issues which explore the detailed relationship of protein structure to function.

Key Features:
* Provides problems and technical solutions
* Includes posttranslational modifications
* Uses synthetic peptides as biological models
* Details mutagenesis and protein engineering
* Covers design of protein structure and function ... Read more

Book Description

Details analytical methods for the determination of mercury * examines the physiology and toxicology of mercury * evaluates the effects of mercury on the immune system and the inhibition of brain tubulin-GTP interactions by mercury * highlights the interactions of mercury with nucleic acids and proteins * discusses the characterization of bacterial mercury resistance genes * and much more. ... Read more