Book Description

This new edition of Bioenergetics presents a clear and up-to-date explanation of the chemiosmotic theory and covers mitochondria, bacteria, and chloroplasts. It takes account of the many newly determined structures, such as ATP synthase and the two photosystems of photosynthesis, that provide molecular insight into chemiosmotic energy transduction. This edition includes additional color figures of protein structures and many newly drawn illustrations designed to enable the reader to grasp the fundamental insights that are derived from knowing the structure. Every chapter has been extensively revised and updated and a new chapter on the study of the bioenergetics of mitochondria in the intact cell is included to satisfy the enormous interest in this topic. Written for students and researchers alike, this book is the most current text on the chemiosmotic theory and membrane bioenergetics available.

Key Features
* Chapter on the study of bioenergetics of mitochondria in the intact cell
* Appendix listing protein structure resources
* Additional colour plates of protein structures
* Many newly drawn illustrations
* Website ... Read more

Book Description

Hardbound. Skeletal muscle development is perhaps one of the best understood processes at the molecular, cellular and organismal level due in large part to the fact that primary myogenic cells (myoblasts) will grow and subsequently differentiate into myotubes in culture.With the advent of reverse mouse genetics, many of the observations gained through the study of myogenic cells in vitro have been directly tested in vivo.What has emerged is a complex but cohesive story of how myogenic cells are initially specified in the vertebrate embryo and how muscle fibers ultimately achieve their respective identities (i.e. fast versus slow) to perform their function.This collection of chapters is focused on these developments.The book discusses old and new directions for the skeletal muscle field and points out directions where the field may eventually progress. ... Read more

Book Description

Cell culture techniques are invaluable to the modern researcher but difficult to carry out successfully. As part of the series of Handbooks in Practical Animal Cell Biology, this volume offers a concise practical guide to the basic essentials of the technique. Researchers new to cell culture will find a clear explanation of the essential equipment of a tissue culture facility, including tissue culture media and sera. It describes methods for growing suspension and adhesion cultures, including how to store cells and prepare primary cultures from cells.For those already culturing cells, the handbook will act as a handy reference to the basic techniques.The essence of the book is to deal with the generalities of cell culture and to give a grasp of the basic concepts before involvement in more specialized work in the field. Ideal for anyone moving into tissue cell culture techniques or looking for a concise reference book. ... Read more

Reviews (1)

From Book to Bench-Cell Culturing Made Easy
Whether a first year college student or a seasoned researcher, General Techniques of Cell Culture is a must. GTCC simplifies the complicated world of cell culturing without insulting. The authors, Maureen A. Harrison and Ian F. Rae, make no assumptions about their readers, and take the task of explaining large amounts of scientific information in an organized and easy to read format. This handbook goes beyond general lab techniques and provides not only the "how-to", but also gives the developing lab the where at, as it rounds out each section with a directory of services and researchable references. By providing time honored techniques, General Techniques of Cell Culture also takes some of the trial and error out of propagating healthy cells in the lab. This alone should make the transition from book to bench less tedius and more productive. ... Read more

Book Description

In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues, a crucial step for understanding the organization, regulation and function of genes. This popular, hands-on guide has been thoroughly updated to cover the techniques currently in use. ... Read more

Reviews (1)

Exellent and newest protocol
This book gives newest and exellent protocol. Contents are not so big but enough detail to follow experiments. ... Read more

Book Description

This book aims to fill a gap in the evolution of living things.Actually, over the last few decades there has been increasing interestin the astronomical and physicochemical problems involved in the eventswhich determined the appearance of the first cell;for almost twocenturies the evolution of present-day and past species has given riseto a great many investigations and much discussion; moreover, in thelast few years important contributions have come from the discoveriesabout their DNA. By contrast, there has been very little investment inattempts to understand the evolutionary processes which linked the firstcell to the first modern organisms, of which are included even the mostancient fossils. In this field, even simple working hypotheses are oftenlacking. This book discusses at length these missing links and theorigin of the basic structures of modern cells ranging from thepropelling organelles of bacteria and nucleated cells to the organellesthat the nucleated cells acquired by... ... Read more

Book Description

A panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposmes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid. ... Read more

Book Description

Expression systems are in widespread use for cloning specific genes, for synthesizing the encoded proteins for structural and functional analysis, and for large-scale preparation of current use with background information and advice on the merits of each, step-by-step practical protocols, troubleshooting, and details of the latest applications. These two books, the first of four volumes are thoroughly revised and up-dated versions of the original ones published in the Practical Approach series. Together they will offer a complete guide to the major techniques required by modern molecular biology laboratories. ... Read more

Book Description

Bidirectional traffic of macromolecules across the nuclear envelope is an active and essential transport process in all eukaryotic cells. Work on various model systems has led to a tremendous increase in our understanding of nuclear transport in recent years.This volume summarizes our current knowledge of protein and RNA transport into and out of the nucleus. It contains nine up-to-date reviews which cover various aspects of nucleocytoplasmic transport, including the structure and function of the nuclear pore complex, the role of soluble transport factors in protein and RNA transport, and the regulation of protein transport through the nuclear pore. ... Read more

Book Description

The polymerase chain reaction (PCR) is a revolutionary technique for enabling the detection and analysis of minute amounts of nucleic acids. Here, Dr. Eva Harris makes this elegantly simple technique accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users with limited budgets, whether in developing countries or high school labs. ... Read more

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BioHacking Bible
This clear, complete and well illistrated book was designed for labs in developing countries that wish to use PCR technology. It also happens to be the perfect manual for those interested in hacking DNA in their kitchens and basements.

If you're interested in actually working with DNA technology and your resources are limited, this is THE book. ... Read more

Book Description

This is the first single source to present this important topic from the points of view of many international experts. Chapters written by morphologists, biochemists, pharmacologists, and molecular biologists from around the world are brought together to provide an introductory overview to this key component of intracellular signalling. This ideal text/reference will be useful to students of the basic sciences and medicine as well as professional scientists who want to update their knowledge in this field. ... Read more

Book Description

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.

Key Features
* Completely revised and greatly expanded since the publication of the First Edition in 1990
* Unique comprehensive methodological coverage
* In-depth treatment of procedures, including:
* Theoretical foundations
* Critical aspects
* Possible pitfalls
* Written by authors with extensive experience who developed or modified the techniques
* Methods cover cell death and cell cycle analyses
* Practical, handbook-style presentation works in the lab and in the classroom
* Color plates illustrate techniques ... Read more

Book Description

Malaria causes more death and disease than any other parasitic pathogen known today. This multiauthored text covers the important areas of malaria research, particularly focusing on those sectors which are of clinical importance for the understanding of the disease, the parasite, and its vector.
The chapter authors are all leading experts within their own particular fields. The biology and molecular biology of the parasite, the clinical spectrum of the disease, the pathogenesis of malaria, and the immunology and emergence of malaria vaccines are some examples of the scientific spheres that are discussed.
The book is suitable as a text for graduate students and clinicians as well as researchers at universities and companies involved in treating or studying infectious diseases. ... Read more

Book Description

Genomic sequences, now emerging at a rapid rate, are greatly expediting certain aspects of molecular biology. However, in more complex organisms, predicting mRNA structure from genomic sequences can often be difficult. Alternative splicing, the use of alternative promoters, and orphan genes without known analogues can call present difficulties in the predictions of the structure of mRNAs or even in gene detection. Both computational and experimental methods remain useful for recognizing genes and transcript templates, even in sequenced DNA. Methods for producing full-length cDNAs are important for determining the structures of the proteins the mRNA encodes, the positions of promoters, and the considerable regulatory information for translation that may be encoded in the 5' untranslated regions of the mRNA.
Methods for studying levels of mRNA and their changes in different physiological circumstances are rapidly evolving, and the information from this area will rival the superabundance of information derived from genomic sequences. In particular, cDNAs can be prepared even from single cells, and this approach has already yielded valuable information in several areas. To the extent that reliable and reproducible information, both quantitative and qualitative, can be generated from very small numbers of cells, there are rather remarkable possibilities for complementing functional and genetic analysis of developmental patterns with descriptions of changes in mRNAs. Dense array analysis promises to be particularly valuable for the rapid expression pattern of known genes, while other methods such as gel display approaches offer the opportunity of discovering unidentified genes or for investigating species whose cDNAs or genomes have not been studied intensively.
Knowledge of mRNA structure, genomic location, and patterns of expression must be converted into information of the function of the encoded proteins. Each gene can be the subject of years of intensive study. Nevertheless, a number of methods are being developed that use cDNA to predict properties or permit the selective isolation of cDNAs encoding proteins with certain general properties such as selective isolation of cDNAs encoding proteins with certain general properties such as subcellular location. This volume presents an update of a number of approaches relevant to the areas referred to above. The technology in this field is rapidly evolving and these contributions represent a "snapshot in time" of the number of currently available and useful approaches to the problems referred to above.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. ... Read more

Book Description

Here is the most complete guide available to the isolation, analysis, and synthesis of RNA. It covers everything researchers and laboratory workers need to know about the study of gene expression via RNA analysis-from the theory behind the methods, to actual problem-solving techniques.

Step-by-step protocols are presented for each method. A careful presentation of the experimental formalities of these protocols enables specialists and nonspecialists alike to implement the methods easily in the laboratory. Each protocol is accompanied by the theoretical background underlying the experimental procedure and most chapters contain illustrations of typical results and troubleshooting tips.

A Laboratory Guide to RNA offers a straightforward detailed account of experimental procedures, ranging from the isolation of RNA from a variety of cell and tissue types, detection analysis, and quantitation using a range of strategies, to large- and small-scale synthesis of RNA. This unique guide not only covers established procedures such as RNA blotting and nuclease protection, but also the latest protocols for quantitative PCR and differential display. Protocols addressing in situ hybridization are highlighted in an eight-page, full-color section that illustrates the power of the technique for detection of gene expression in tissues and whole organisms.

Featuring contributions from leading research laboratories and the biotechnology field, A Laboratory Guide to RNA: Isolation, Analysis, and Synthesis provides all the methods required for RNA analysis. It is the ideal laboratory guide for research scientists, graduate students, and lab personnel who need a solid reference on the analysis of gene expression at the RNA level. ... Read more

Book Description

Analysis of the neural tissue presents unique and peculiar technical problems encountered in everyday bench work. Numerous books dealing with cellular and molecular protocols for general use in cell biology are available, but few are specifically devoted exclusively to neurobiology. Moreover, the "cross-talk" between researchers with different backgrounds, i.e. histologists, cell and molecular biologists and physiologists, is still quite difficult, and very often one remains somehow "confined" to his or her own specific field of expertise never daring to explore "mysterious" lands unless having the support of a big laboratory beyond. The general idea beyond this project was to put together the contributions from a number of well-known neuroscientists to produce a book that offers a survey of the most updated techniques for the study of nerve cells. After a long time spent doing research in the neuroscience field, and having acquired a good technical background in certain specific fields of neurobiology we have realized how difficult is to be able to step into a different technology. This book endeavors to assist in that goal. ... Read more

Book Description

This text provides a unique combination of succinctly expressed basic concepts of cell growth and cell death with detailed instructions and protocols on how accurately to measure these processes. Practical instructions are accompanied by explanatory material which allows the researcher to choose which particular protocol is most suitable. The methods outlined range from simple techniques such as autoradiography and cell staining, to more complex techniques such as flow cytometry. This book will be essential to laboratory researchers studying the growth, division and dealth of cells. ... Read more

Book Description

Cell Surface Receptors: A Short Course on Theory and Methods, Second Edition is a primer for the study of cell surface receptors. The simplified discussion of methods and their underlying principles removes the usual intimidation caused by the specialized vocabulary or sophisticated mathematics that characterize many of the primary papers in this field. In this way, the basic concepts become emphasized. This volume is a starting point: a textbook as well as a manual to which the investigator can return for a refresher course, when needed. ... Read more

Reviews (1)

Highly readable and very informative
This book describes the mathematics of receptor ligand binding in a very readable fashion.I actually enjoyed reading it! It covers simple and complex binding interactions.It also contains detailed methods forperforming the experiments.I highly recommend it.I am finally going topurchase this book, after wanting to for 10 years. ... Read more

Reviews (2)

available science to undergraduates
Easy to read, easy to understand, easy to follow. 3 main aspects of this wonderfull book yet very rich in its contents. My appreciation goes to the fact that Gary Matthews is able to turn deep information about nerves and muscle cells in a way that we feel a pHD. about the subject. Very clever the way he starts every chapter making us feel we already knew all that stuff anyway it is not so.

An introductory text deserved to be mentioned
What could be worse than sitting in a lecture room without understanding the lecture material and desparately looking for a reference book that will lead you through the topics carefully? Sounds familiar, isn'it? Well, here is a book deserved to be mentioned.

As a medical student, I have come across at least ten textbooks in cell physiology. Many of them are written without bearing in mind who their audiences are. However,"Gary Matthews';Cellular Physiology of Nerve and Muscle" has approached the topics in the student's shoes and talked OUR LANGUAGE. Instead of throwing you the formulae, he would try to take you through the idea behind it such as how they are dereived and answers your "WHYS". For instance, he takes his reader step by step to how the membrane potential is determined - really impressive.

A little bit of understanding in basic biology, chemistry and physics would allow you read through the book comfortably. It is true that this book is aiming at a beginner group; it is however, a fantasic teaching tool for all lecturers (to draw examples) and those who would like to fully understand the fundamental science about synapses and neurotransmission.
... Read more